Komatsu T, Nishide H, Tsuchida E
Department of Polymer Chemistry Waseda University, Tokyo, Japan.
Artif Cells Blood Substit Immobil Biotechnol. 1994;22(3):855-60. doi: 10.3109/10731199409117921.
Triglyceride microsphere emulsified with phospholipid derivative of heme (5,10,15,20-tetrakis[alpha,alpha,alpha,alpha-o-[2,2-dimethyl-20- [2-(trimethylammonioethoxy) phosphonatoxy]eicosanamido]phenyl]porphinatoiron(II); lipidheme) provides a totally synthetic artificial red cell (lipidheme-microsphere; LH-M). Its structure, solution properties and O2 binding ability are described. The particle diameter of the LH-M was ca. 90 nmø elucidated by electron microscopy. Viscosity of the LH-M suspension (approximately 1.5 cP) was much lower than that of human blood and the viscosity of mixed system of LH-M/human blood (1/1(v/v)) was 2.5 cP. Specific gravity, osmotic pressure, and colloid osmotic pressure of the LH-M suspension also satisfied the physiological needs. The LH-M can bind O2 reversibly in response to O2 pressure (P50(O2): 41 torr (pH 7.4, 37 degrees C)). O2 solubility of the LH-M was more than that of human blood caused by its high heme concentration.
用血红素的磷脂衍生物（5,10,15,20-四[α,α,α,α-o-[2,2-二甲基-20-[2-(三甲基铵乙氧基)膦酰氧基]二十碳酰胺基]苯基]卟啉铁(II)；脂质血红素）乳化的甘油三酯微球提供了一种完全合成的人造红细胞（脂质血红素微球；LH-M）。描述了其结构、溶液性质和氧气结合能力。通过电子显微镜阐明，LH-M的粒径约为90 nmø。LH-M悬浮液的粘度（约1.5 cP）远低于人血，LH-M/人血混合体系（1/1(v/v)）的粘度为2.5 cP。LH-M悬浮液的比重、渗透压和胶体渗透压也满足生理需求。LH-M可根据氧气压力可逆地结合氧气（P50(O2)：41托（pH 7.4，37℃））。由于其高血红素浓度，LH-M的氧气溶解度高于人血。
