Yu W P, Chang T M
Artificial Cells and Organs Research Centre Faculty of Medicine, McGill University, Montreal, Quebec, Canada.
Artif Cells Blood Substit Immobil Biotechnol. 1994;22(3):889-93. doi: 10.3109/10731199409117926.
We used biodegradable polymers like polylactic acid, poly-isobutylcyanoacrylate and others to prepare nanocapsules containing hemoglobin. We prepared the optimal mean diameters of between 0.08 to 0.12 micron (80-120 nm). They are spherical and homogeneous. The membrane thickness is 0.005-0.015 micron (5-15nm). With different formulation, the hemoglobin contents in the particles may be varied from 30-45%. Phospholipid is not required in bovine hemoglobin. It is required for human hemoglobin in order to retain cofactors required for optimal P50. The P50 of the biodegradable polymer membrane containing bovine hemoglobin was between 27-29 mmHg. This is the same P50 as bovine hemoglobin used in the preparation. Thus the procedure of preparation did not damage hemoglobin.
我们使用了可生物降解的聚合物，如聚乳酸、聚异丁基氰基丙烯酸酯等，来制备含有血红蛋白的纳米胶囊。我们制备的纳米胶囊的最佳平均直径在0.08至0.12微米（80 - 120纳米）之间。它们呈球形且均匀。膜厚度为0.005 - 0.015微米（5 - 15纳米）。通过不同的配方，颗粒中的血红蛋白含量可在30% - 45%之间变化。牛血红蛋白不需要磷脂。人血红蛋白则需要磷脂以保留最佳P50所需的辅因子。含有牛血红蛋白的可生物降解聚合物膜的P50在27 - 29 mmHg之间。这与制备过程中使用的牛血红蛋白的P50相同。因此，制备过程没有损害血红蛋白。
