Yu W P, Chang T M
Department of Physiology, McGill University Montreal, Quebec, Canada.
Artif Cells Blood Substit Immobil Biotechnol. 1996 May;24(3):169-83. doi: 10.3109/10731199609117433.
Biodegradable polymer membrane nanocapsules containing hemoglobin have been prepared with phase separation and polymer precipitation methods. We have used different polymers including polylactic acid and polyisobutyl-cyanoacrylate. The mean diameter of the hemoglobin nanocapsules can be as low as 0.07 mu and up to 0.39 mu dependent on the method of preparation. The concentration of the hemoglobin in the suspension can be up to 11 gm/dl, as compared to 15 gm/dl in whole blood. The viscosity of the preparation is slightly lower than that of human blood. In vitro tests show that P50 of these nanocapsules containing bovine hemoglobin is identical to free bovine hemoglobin solution. The surface of the nanocapsules can be modified for example with polyethylene glycol(PEG) or with PEG 2000 PE. Preliminary in vivo tests show that surface modified nanocapsules containing hemoglobin can survive longer in the circulation.
采用相分离和聚合物沉淀法制备了含血红蛋白的可生物降解聚合物膜纳米胶囊。我们使用了不同的聚合物，包括聚乳酸和聚异丁基氰基丙烯酸酯。血红蛋白纳米胶囊的平均直径可低至0.07微米，高至0.39微米，这取决于制备方法。悬浮液中血红蛋白的浓度可达11克/分升，而全血中的浓度为15克/分升。制剂的粘度略低于人体血液。体外试验表明，这些含牛血红蛋白的纳米胶囊的P50与游离牛血红蛋白溶液相同。纳米胶囊的表面可以用例如聚乙二醇（PEG）或PEG 2000 PE进行修饰。初步体内试验表明，表面修饰的含血红蛋白纳米胶囊在循环系统中的存活时间更长。
