Tsarev V N, Ushakov R V, Vaneeva N P, Iastrebova N E, Ushakova T V
Klin Lab Diagn. 1993 Sep-Oct(5):11-5.
A test system for enzyme immunoassay of antibodies to Bacteroides melaninogenicus has been developed. The authors describe a method for the preparation of the protein and polysaccharide antigens with the use of ultrasonic, lysozyme, and beta-naphthol treatment. A total of 105 blood serum samples, 46 samples of gingival blood and gingival fluid from patients with maxillofacial phlegmons and periodontitis were examined, making use of the 1 +/- 3 omega index (from the values in healthy donors). The results evidence statistically significant difference in antibody levels of patients from whom B. melaninogenicus were isolated and of those with the negative results of bacteriologic analysis. The titers of antibodies to B. melaninogenicus in the blood and gingival fluid of patients with periodontitis were in good correlation.
已开发出一种用于酶免疫测定产黑素拟杆菌抗体的检测系统。作者描述了一种使用超声、溶菌酶和β-萘酚处理来制备蛋白质和多糖抗原的方法。利用1±3ω指数（来自健康供体的值），对105份血清样本、46份来自颌面部蜂窝织炎和牙周炎患者的牙龈血和牙龈液样本进行了检测。结果表明，分离出产黑素拟杆菌的患者与细菌学分析结果为阴性的患者的抗体水平存在统计学上的显著差异。牙周炎患者血液和牙龈液中产黑素拟杆菌抗体的滴度具有良好的相关性。
