Labbé M, Delamare N, Pepersack F, Crokaert F, Yourassowsky E
J Clin Pathol. 1980 Dec;33(12):1189-92. doi: 10.1136/jcp.33.12.1189.
A new diagnostic kit, which contains a polyvalent antiserum for either Bacteroides fragilis or Bacteroides melaninogenicus, was tested for reliability and specificity on 146 clinical samples of different origin. A correlation between the culture and immunofluorescence was observed for B. fragilis in 87.39% of cases and for B. melaninogenicus in 81.48% of cases. When pure cultures were tested, aerobically as well as anaerobically, false-positive reactions were observed with staphylococci and Clostridium ramosum spores. The well-defined morphology of these bacteria and spores allows for the elimination of any diagnostic error. The method is rapid, and the margin for error is limited. The test gives a semiquantitative idea of the number of bacteroides organisms present in the clinical specimens even in the presence of a mixed flora.
一种新的诊断试剂盒,其中包含用于脆弱拟杆菌或产黑色素拟杆菌的多价抗血清,在146份不同来源的临床样本上进行了可靠性和特异性测试。在87.39%的病例中观察到脆弱拟杆菌培养与免疫荧光之间的相关性,在81.48%的病例中观察到产黑色素拟杆菌培养与免疫荧光之间的相关性。当对纯培养物进行需氧和厌氧测试时,观察到葡萄球菌和多枝梭菌孢子出现假阳性反应。这些细菌和孢子明确的形态有助于消除任何诊断误差。该方法快速,误差范围有限。即使存在混合菌群,该测试也能对临床标本中拟杆菌的数量给出半定量概念。