Candotti D, Chappey C, Rosenheim M, M'Pelé P, Huraux J M, Agut H
Laboratoire de Bactériologie-Virologie, CNRS EP 57, CERVI, Hôpital de la Pitié-Salpêtrière, Paris, France.
C R Acad Sci III. 1994 Feb;317(2):183-9.
The overlapping region of gag and pol genes of human immunodeficiency virus type 1 (HIV-1) also called transframe region, contains the frameshift locus from gag to pol. This region encodes both the protein p6, the function of which remains unclear, and a putative transframe protein covently linked to the N-terminus of the viral protease within Gag/Pol protein precursor. We have investigated the variability of the transframe region among nine HIV-1 isolates obtained from Congolese AIDS patients. Nucleotide sequences were determined using the polymerase chain reaction and the direct sequencing of amplified products. The sequences of Congolese isolates markedly differed from one another and from other reference HIV-1 strains by both insertion-deletion events and numerous base substitutions. Several putative cleavage sites of precursor polypeptides were modified. When compared to consensus ones the amino acid sequences of p6 protein were very different among divergent HIV-1 isolates, except for a limited group of 10 conserved amino acids.
人类免疫缺陷病毒1型（HIV-1）的gag基因和pol基因的重叠区域，也称为移码区，包含从gag到pol的移码位点。该区域既编码功能尚不清楚的p6蛋白，也编码一种与Gag/Pol蛋白前体中病毒蛋白酶N端共价连接的推定移码蛋白。我们研究了从刚果艾滋病患者中获得的9株HIV-1分离株移码区的变异性。使用聚合酶链反应和扩增产物的直接测序来确定核苷酸序列。刚果分离株的序列彼此之间以及与其他参考HIV-1毒株在插入-缺失事件和大量碱基替换方面都有显著差异。前体多肽的几个推定切割位点发生了改变。与共有序列相比，不同的HIV-1分离株中p6蛋白的氨基酸序列差异很大，只有一组10个保守氨基酸除外。
