Gachie F, Carreau S
Laboratoire de Biochimie-IRBA, URA CNRS 609, Université de Caen, France.
C R Acad Sci III. 1994 Feb;317(2):190-3.
In Percoll purified Leydig cells from mature rat incubated in Ham F12/DME medium, we have demonstrated that the testosterone production (1 ng/10(6) Leydig cells/3 h) is increased 9 fold in presence of a saturating amount of oLH but diminished when larger doses of gonadotropin are used. However, if SCM or STM is added together with oLH to the Leydig cell incubation medium, a further increase (69%) of testosterone output is noticed. Obviously, when the testosterone production is low consecutive to a desensitizing dose of oLH, the STM or SCM improves at least 2 fold the steroid synthesis. Therefore, the paracrine factor (or factors) presents in SCM (or STM) which is testis specific and acts in synergy with dbcAMP and 22R-hydroxycholesterol, uses an alternative pathway to improve rat Leydig cell testosterone production since its biological effect is highly potent in absence and in presence of oLH. Therefore, these data bring new insights in the regulation of rat Leydig cell steroidogenesis through cAMP-independent channel suggesting either an increase of the cholesterol side chain cleavage activity and/or availability of the cholesterol to mitochondria.
在汉姆F12/DME培养基中培养的经Percoll纯化的成熟大鼠睾丸间质细胞中，我们已经证明，在存在饱和量的促黄体生成素（oLH）时，睾酮生成量（1纳克/10⁶个睾丸间质细胞/3小时）增加9倍，但当使用更大剂量的促性腺激素时则减少。然而，如果将血清条件培养基（SCM）或睾丸条件培养基（STM）与oLH一起添加到睾丸间质细胞孵育培养基中，会发现睾酮产量进一步增加（69%）。显然，当由于oLH脱敏剂量导致睾酮生成量较低时，STM或SCM至少能使类固醇合成提高2倍。因此，SCM（或STM）中存在的旁分泌因子与二丁酰环磷腺苷（dbcAMP）和22R-羟基胆固醇协同作用，采用替代途径来提高大鼠睾丸间质细胞的睾酮生成，因为其生物学效应在不存在和存在oLH时都非常有效。因此，这些数据为通过不依赖cAMP的途径调节大鼠睾丸间质细胞类固醇生成带来了新的见解，这表明胆固醇侧链裂解活性增加和/或胆固醇向线粒体的可用性增加。
