Bilinska B, Genissel C, Carreau S
Laboratoire de Biochimie-IRBA, EP CNRS 009, Université de Caen, France.
Biol Cell. 1997 Oct;89(7):435-42. doi: 10.1016/s0248-4900(97)89314-8.
In Percoll purified Leydig cells from mature rat we have demonstrated that the basal testosterone production (9.5 ng/10(6) Leydig cells/24 h) is increased 10-fold in presence of a saturating amount of hCG (1 IU/mL) and diminished in a dose-related manner when larger concentrations of gonadotropin are used to reach 14 ng/10(6) Leydig cells for 50 IU of hCG. If 40% (v/v) seminiferous tubule medium (STM) is added together with hCG (1 IU/mL) to the incubation medium, a further increase (62%) of testosterone output is noticed. Obviously, when the testosterone production is low as a consequence of a higher dose of hCG (50 IU/mL), the STM (80%) improves the steroid synthesis five-fold (67.4 ng). Concerning the cytoskeletal components (microtubules, intermediate filaments and microfilaments) which have been examined in presence or absence of hCG and STM, we have found a rearrangement of cytoskeletal elements as well as cell-shape changes in relation with hormonal activity of the cells. The most prominent alterations of cytoskeletal elements have been observed after 24 h of incubation with 1 IU/mL of hCG added together with 80% of STM. The obtained results suggest that paracrine factor(s) presents in STM and acting in synergy with LH/hCG generate(s) the rearrangement of cytoskeletal structures which, in turn, facilitates the availability of cholesterol for the mitochondria and finally enhances the testosterone production in the rat Leydig cells.
在从成熟大鼠中通过Percoll纯化得到的睾丸间质细胞中，我们已经证明，基础睾酮分泌量（9.5 ng/10⁶个睾丸间质细胞/24小时）在存在饱和量的人绒毛膜促性腺激素（hCG，1 IU/mL）时增加10倍，而当使用更高浓度的促性腺激素以达到50 IU的hCG使睾酮分泌量达到14 ng/10⁶个睾丸间质细胞时，基础睾酮分泌量会以剂量相关的方式减少。如果将40%（v/v）的生精小管培养基（STM）与hCG（1 IU/mL）一起添加到孵育培养基中，会发现睾酮分泌量进一步增加（62%）。显然，当由于更高剂量的hCG（50 IU/mL）导致睾酮分泌量较低时，STM（80%）可使类固醇合成提高5倍（67.4 ng）。关于在有或无hCG和STM存在的情况下检测的细胞骨架成分（微管、中间丝和微丝），我们发现细胞骨架成分发生了重排，并且细胞形状也随着细胞的激素活性发生了变化。在用1 IU/mL的hCG与80%的STM一起孵育24小时后，观察到细胞骨架成分最显著的改变。所得结果表明，STM中存在的旁分泌因子与促黄体生成素/人绒毛膜促性腺激素协同作用，导致细胞骨架结构重排，这反过来又促进了胆固醇向线粒体的供应，最终提高了大鼠睾丸间质细胞中的睾酮分泌量。
