Jacque C, Tchélingérian J L
INSERM U-134, Hôpital de la Salpêtrière, Paris, France.
C R Seances Soc Biol Fil. 1994;188(1):47-57.
This report provides the immunohistochemical demonstration of the appearance of tumor necrosis factor alpha and interleukin-1 alpha in neuronal cells of different regions of the brain after a surgical injury to the hippocampus. We also demonstrate, by an in situ hybridization technique using a digoxigenin-labeled probe, the induction of TNF alpha mRNA in these neurons. Control lesions in brain areas such as the striatum, the parietal cortex or the cerebellum do not induce the neuronal expression of these cytokines. We hypothesize that cytokines production by neurons may be related with neurotrophin synthesis and restoration of homeostasis in an attempt to promote survival and regeneration of specific neuronal cell populations after a lesion to the hippocampus.
本报告提供了海马体手术损伤后，大脑不同区域神经元细胞中肿瘤坏死因子α和白细胞介素-1α出现的免疫组织化学证明。我们还通过使用地高辛标记探针的原位杂交技术，证明了这些神经元中肿瘤坏死因子α mRNA的诱导。纹状体、顶叶皮质或小脑等脑区的对照损伤不会诱导这些细胞因子的神经元表达。我们推测，神经元产生细胞因子可能与神经营养因子的合成以及内环境稳态的恢复有关，试图促进海马体损伤后特定神经元细胞群的存活和再生。
