Shu J, Benedict S H, Chan M A
Department of Pharmacology and Toxicology, Higuchi Biosciences Center, University of Kansas, Lawrence 66045.
Cell Immunol. 1994 Dec;159(2):170-83. doi: 10.1006/cimm.1994.1305.
One component of the B cell activation cascade is the induction of the protooncogene c-fos. Data presented here demonstrate that stimulation of mIgM-bearing cells with either anti-IgM or the combination of PDB plus ionomycin generated comparable levels of c-fos RNA. Furthermore, a synergistic response was observed when the cells were treated with selected concentrations of anti-IgM plus either PDB or ionomycin. In contrast, stimulation of mIgG-bearing B cells with anti-IgG did not induce the c-fos RNA levels that were seen when these cells were treated with the combination of PDB plus ionomycin. Treatment of mIgG-bearing cells with only the combination of anti-IgG plus ionomycin yielded a synergistic response and anti-IgG plus PDB did not. Thus, induction of c-fos RNA appears to be different in mIgM- and mIgG-bearing B cells after stimulation through mIg.
B细胞激活级联反应的一个组成部分是原癌基因c-fos的诱导。此处呈现的数据表明，用抗IgM或PDB加离子霉素的组合刺激携带mIgM的细胞会产生相当水平的c-fos RNA。此外，当用选定浓度的抗IgM加PDB或离子霉素处理细胞时，观察到了协同反应。相比之下，用抗IgG刺激携带mIgG的B细胞并不会诱导出在用PDB加离子霉素组合处理这些细胞时所观察到的c-fos RNA水平。仅用抗IgG加离子霉素的组合处理携带mIgG的细胞会产生协同反应，而抗IgG加PDB则不会。因此，通过mIg刺激后，携带mIgM和mIgG的B细胞中c-fos RNA的诱导情况似乎有所不同。
