Chan M A, McGrath T H, Gelfand E W
Department of Pharmacology and Toxicology, University of Kansas, Lawrence 66045.
Cell Immunol. 1993 Feb;146(2):300-12. doi: 10.1006/cimm.1993.1028.
We have targeted the specific surface IgM-bearing subset of human B lymphocytes within a mixed population and investigated cell cycle activation in these cells stimulated with phorbol ester and antibody directed against surface immunoglobulin. Stimulation with phorbol 12,13-dibutyrate (PDB) in combination with anti-IgM led to induction of the state of competence followed by progression to proliferation of the surface IgM-bearing cells. In contrast, sequential stimulation with PDB and anti-IgM in either order did not induce either competence or progression. Brief exposure to both stimuli for 30 min did not induce significant proliferation, but did induce the state of competence such that the cells progressed to DNA synthesis after incubation with PDB alone. Furthermore, a competence-related gene, c-fos, was induced in cells that did not become fully competent. Thus, induction of competence to proliferate required simultaneous delivery of two signals whereas actual progression to proliferation by competent cells was accomplished with only one signal.
我们已针对混合群体中携带特异性表面IgM的人类B淋巴细胞亚群，并研究了这些细胞在用佛波酯和针对表面免疫球蛋白的抗体刺激时的细胞周期激活情况。用佛波12,13 - 二丁酸酯（PDB）与抗IgM联合刺激导致感受态状态的诱导，随后携带表面IgM的细胞进入增殖阶段。相比之下，以任一顺序先后用PDB和抗IgM刺激均未诱导出感受态或增殖。短暂暴露于两种刺激30分钟未诱导出显著增殖，但确实诱导出了感受态状态，使得细胞在单独与PDB孵育后进入DNA合成阶段。此外，在未完全获得感受态的细胞中诱导出了一个与感受态相关的基因c - fos。因此，诱导增殖的感受态需要同时传递两个信号，而感受态细胞实际进入增殖阶段仅需一个信号。
