Zhao Z S, Calder V L, McLauchlan M, Lightman S L
Department of Clinical Science, Institute of Ophthalmology, London, United Kingdom.
Cell Immunol. 1994 Dec;159(2):220-34. doi: 10.1006/cimm.1994.1309.
Retinal soluble antigen (S-Ag) and purified protein derivative (PPD)-specific T cell lines established from Lewis rats were used to study the pattern of lymphokine expression to see if it varied with the inducing stimulus. Lymphokine mRNA expression was detected by PCR combined with Southern analysis after 6-hr stimulation and protein secretion assessed by bioassays at 24 hr poststimulation. S-Ag-specific T cell lines when stimulated with antigen expressed IL-2, IFN-gamma, and IL-4 mRNA, whereas only IL-2 and IFN-gamma could be detected in the supernatants. This is in contrast to the findings after stimulation of the PPD cell lines with PPD where IL-4 could be detected in the supernatants. The time course studies (3, 6, 24, 48, and 72 hr) with one of the S-Ag-specific T cell lines showed that S-Ag activation did not induce any detectable IL-4 bioactivity. However, when the S-Ag T cell line was stimulated by Con A or PMA, IL-4 was detected in the supernatants following Con A activation, suggesting that the way in which the T cell is activated has an effect on its resultant lymphokine secretion.
利用从Lewis大鼠建立的视网膜可溶性抗原（S-Ag）和纯化蛋白衍生物（PPD）特异性T细胞系，研究淋巴因子表达模式，以观察其是否随诱导刺激而变化。在6小时刺激后，通过PCR结合Southern分析检测淋巴因子mRNA表达，并在刺激后24小时通过生物测定评估蛋白分泌。用抗原刺激S-Ag特异性T细胞系时，表达IL-2、IFN-γ和IL-4 mRNA，而在上清液中仅能检测到IL-2和IFN-γ。这与用PPD刺激PPD细胞系后在上清液中能检测到IL-4的结果形成对比。对其中一个S-Ag特异性T细胞系进行的时间进程研究（3、6、24、48和72小时）表明，S-Ag激活未诱导任何可检测到的IL-4生物活性。然而，当S-Ag T细胞系用刀豆蛋白A（Con A）或佛波酯（PMA）刺激时，Con A激活后在上清液中检测到IL-4，这表明T细胞的激活方式对其最终的淋巴因子分泌有影响。
