Devine L, Lightman S, Greenwood J
Department of Clinical Science, Institute of Ophthalmology, University College London, United Kingdom.
Cell Immunol. 1996 Mar 15;168(2):267-75. doi: 10.1006/cimm.1996.0075.
The migration of lymphocytes through monolayers of rat retinal pigment epithelium (RPE) and retinal vascular endothelium, which form the posterior and anterior blood-retinal barrier (BRB) respectively, was investigated in vitro. After a 4-hr assay the migration of untreated peripheral lymph node (PLN) cells through RPE monolayers was negligible (<1%) with only a small increase found after activation of the PLN cells with concanavalin A or by cross-linking CD3. Activation of the RPE with IFN-gamma augmented migration with maximal PLN cell migration being achieved with a combination of CD3 cross-linking and IFN-gamma activation (17% migration). The highest level of lymphocyte migration was observed with three CD4+ antigen-specific T cell lines specific for purified protein derivative (PPD; 33% migration), ovalbumin (OA; 31%), and S-antigen (S-Ag; 57%). Migration of both untreated and Con A-activated PLN cells through retinal endothelial cells (EC) from PVG rats was negligible, whereas the migration of the antigen-specific T cell lines was 23, 29 and 23% for PPD, OA, and S-Ag lines, respectively. Migration of these cell lines through retinal endothelium derived from Lewis rats was significantly greater (44% for PPD, 39% for OA, and 39% for S-Ag) which corresponded with a greater expression of ICAM-1 on the EC.
在体外研究了淋巴细胞通过大鼠视网膜色素上皮（RPE）单层和视网膜血管内皮细胞单层的迁移情况，这两种细胞单层分别形成了后部和前部血视网膜屏障（BRB）。经过4小时的检测，未处理的外周淋巴结（PLN）细胞通过RPE单层的迁移可以忽略不计（<1%），在用伴刀豆球蛋白A激活PLN细胞或通过交联CD3后，迁移仅有少量增加。用γ干扰素激活RPE可增强迁移，在用CD3交联和γ干扰素激活联合处理时，PLN细胞迁移达到最大值（17%迁移）。对于三种针对纯化蛋白衍生物（PPD；33%迁移）、卵清蛋白（OA；31%）和S抗原（S-Ag；57%）的CD4+抗原特异性T细胞系，观察到了最高水平的淋巴细胞迁移。未处理的和经刀豆蛋白A激活的PLN细胞通过PVG大鼠的视网膜内皮细胞（EC）的迁移可以忽略不计，而对于PPD、OA和S-Ag细胞系，抗原特异性T细胞系的迁移分别为23%、29%和23%。这些细胞系通过Lewis大鼠来源的视网膜内皮的迁移明显更高（PPD为44%，OA为39%，S-Ag为39%），这与EC上细胞间黏附分子-1（ICAM-1）的更高表达相对应。
