Manzo C, Ferraiuolo C
Oncologia Sperimentale C,-Immunologia, Istituto Nazionale Tumori Fondazione Pascale, Napoli, Italy.
Clin Exp Immunol. 1994 Dec;98(3):508-12. doi: 10.1111/j.1365-2249.1994.tb05520.x.
MLR is a 6-day routine proliferation assay used to demonstrate HLA disparity between two individuals. However, on day 2 only a negligible proliferation can be detected. In contrast, we found a significant increase of the extent of proliferation if an anti-CD3 IgA antibody was present in the culture. Since the CD3 proliferation pathway cannot be triggered by an antibody of IgA isotype the result was interpreted as a consequence of a synergism between the allogeneic stimulus and the anti-CD3 antibody effect. The level of this proliferation well correlates with the HLA difference, thus suggesting the use of this methodology for a rapid detection of the allogeneic reaction.
混合淋巴细胞反应（MLR）是一种为期6天的常规增殖试验，用于证明两个人之间的HLA差异。然而，在第2天只能检测到可忽略不计的增殖。相比之下，我们发现如果培养物中存在抗CD3 IgA抗体，增殖程度会显著增加。由于IgA同种型抗体不能触发CD3增殖途径，因此该结果被解释为同种异体刺激与抗CD3抗体效应之间协同作用的结果。这种增殖水平与HLA差异密切相关，因此表明可使用这种方法快速检测同种异体反应。
