Rehany U, Waisman M
Department of Ophthalmology Western Galilee Regional Medical Center, Nahariya, Israel.
Cornea. 1994 Sep;13(5):447-53. doi: 10.1097/00003226-199409000-00013.
Immunologic rejection is the main cause of corneal graft failure, especially in vascularized corneal beds. The purpose of this study was to investigate the effect of systemic Cyclosporine-A (CsA) on the survival of corneal allografts in heavily vascularized rabbit corneal beds, following alkali burn. Heavy corneal vascularization was induced in one eye of 20 rabbits by alkali burn. Forty-five days later, penetrating keratoplasty was performed in all the heavily vascularized corneas. Twenty-five mg/kg/day of CsA was intramuscularly administered to 10 rabbits for 30 days. The other 10 rabbits were treated with the solvent without CsA and were used as a matched control group. The results show a significant difference in corneal allograft survival between the two groups. All corneal grafts in the untreated group were intensely rejected and vascularized within 3 weeks. Nine of the 10 corneal transplants, in the CsA-treated group, remained transparent without signs of immunologic rejection for > 180 days. In one corneal transplant, minor signs of rejection occurred. We suggest that CsA, when given systemically, is a potent drug in the prevention of immunologic rejection in high-risk corneal transplantations, such as allografts, in heavily vascularized corneas following alkali burn.
免疫排斥是角膜移植失败的主要原因，尤其是在角膜血管化的情况下。本研究的目的是探讨全身应用环孢素A（CsA）对碱烧伤后重度血管化兔角膜床同种异体角膜移植存活的影响。通过碱烧伤诱导20只兔的一只眼睛发生重度角膜血管化。45天后，对所有重度血管化角膜进行穿透性角膜移植术。10只兔每天肌肉注射25mg/kg的CsA，持续30天。另外10只兔用不含CsA的溶剂治疗，作为配对对照组。结果显示两组之间角膜同种异体移植存活存在显著差异。未治疗组的所有角膜移植在3周内均发生强烈排斥并血管化。CsA治疗组的10只角膜移植中有9只在超过180天内保持透明，无免疫排斥迹象。在一只角膜移植中出现了轻微的排斥迹象。我们认为，全身应用CsA是预防高风险角膜移植（如碱烧伤后重度血管化角膜的同种异体移植）免疫排斥的有效药物。
