Chong B S, Owadally I D, Pitt Ford T R, Wilson R F
Department of Conservative Dental Surgery, United Medical and Dental Schools, Guy's Hospital, London, England.
Endod Dent Traumatol. 1994 Jun;10(3):129-33. doi: 10.1111/j.1600-9657.1994.tb00537.x.
The cytotoxicity of potential retrograde root-filling materials was assessed using the Millipore filter method. A light-cured glass ionomer cement, Vitrebond (VB), and three reinforced zinc oxide-eugenol cements, Kalzinol, IRM and ethoxybenzoic acid (EBA) cement, were compared with amalgam. Twenty standardized pellets of each material, 10 fresh and 10 aged by storage in sterile distilled water for 72 h were produced. Ten filters were included as controls: five filters with a cell monolayer, but without test specimens, and another five without cells but with test specimens. Fresh IRM exhibited the most pronounced cytotoxic effect and the difference was statistically significant compared with all the other materials. There was no statistically significant difference between fresh VB, Kalzinol, EBA cement and amalgam. When aged, Kalzinol was the most cytotoxic and the difference was statistically significant compared with the other materials. Aged IRM was the second most cytotoxic material and this too was statistically different. The cytotoxicity of VB, EBA, and amalgam did not differ significantly from one another.
采用微孔滤膜法评估潜在的逆行根管充填材料的细胞毒性。将一种光固化玻璃离子水门汀（Vitrebond，VB）、三种增强型氧化锌丁香酚水门汀（Kalzinol、IRM和乙氧基苯甲酸（EBA）水门汀）与汞合金进行比较。制备了每种材料的20个标准化小球，其中10个新鲜小球，另外10个通过在无菌蒸馏水中储存72小时进行老化处理。设置10个滤膜作为对照：5个带有细胞单层但没有测试样本的滤膜，以及另外5个没有细胞但有测试样本的滤膜。新鲜的IRM表现出最明显的细胞毒性作用，与所有其他材料相比差异具有统计学意义。新鲜的VB、Kalzinol、EBA水门汀和汞合金之间没有统计学上的显著差异。老化后，Kalzinol的细胞毒性最强，与其他材料相比差异具有统计学意义。老化的IRM是细胞毒性第二大的材料，这也具有统计学差异。VB、EBA和汞合金的细胞毒性彼此之间没有显著差异。
