Torabinejad M, Hong C U, Pitt Ford T R, Kettering J D
Department of Endodontics, School of Dentistry, Loma Linda University, Loma Linda, California, USA.
J Endod. 1995 Oct;21(10):489-92. doi: 10.1016/s0099-2399(06)80518-2.
Leakage and scanning electron microscopic studies have shown that a mineral trioxide aggregate (MTA) used as root end filling material permits significantly less leakage and has better adaptation than other commonly used root end filling materials. Because these materials are in contact with the periradicular tissues, their cytotoxicity should be evaluated before in vivo tests. Using the agar overlay and radiochromium methods, the cytotoxicity of amalgam, Super EBA, IRM, and the MTA was evaluated. Statistical analysis of the data from the agar overlay technique shows that freshly mixed and set amalgam were significantly less toxic than the rest of tested materials (p< 0.00005). Fresh and set MTA ranked second when tested for cytotoxicity with this technique. Similar statistical tests revealed a significant statistical difference between the toxicity of freshly mixed and set materials after 24 h of incubation with radiochromium-labeled mouse L929 cells. The degree of cytotoxicity of fresh and set materials was MTA least toxic followed by amalgam, Super EBA, and IRM. Based on the results of the cell culture methods used in this study it appears that MTA is a potential root end filling material and warrants further in vivo evaluations.
渗漏和扫描电子显微镜研究表明，用作根尖充填材料的三氧化矿物凝聚体（MTA）相比其他常用的根尖充填材料，渗漏显著更少且适应性更好。由于这些材料与根尖周组织接触，在进行体内试验之前应评估其细胞毒性。采用琼脂覆盖法和放射性铬法，对汞合金、Super EBA、IRM和MTA的细胞毒性进行了评估。来自琼脂覆盖技术的数据的统计分析表明，新调和凝固的汞合金的毒性显著低于其他受试材料（p<0.00005）。用该技术检测细胞毒性时，新鲜和凝固的MTA排名第二。类似的统计测试显示，在用放射性铬标记的小鼠L929细胞孵育24小时后，新调和凝固材料的毒性之间存在显著的统计学差异。新鲜和凝固材料的细胞毒性程度为MTA毒性最小，其次是汞合金、Super EBA和IRM。基于本研究中使用的细胞培养方法的结果，MTA似乎是一种潜在的根尖充填材料，值得进一步进行体内评估。
