Gupta G, Srivastava A, Setty B S
Division of Endocrinology, Central Drug Research Institute, Lucknow, India.
Endocr Res. 1994 Aug;20(3):275-90. doi: 10.1080/07435809409035864.
The activities of nine enzymes of the TCA cycle were estimated in the initial segment, caput, corpus and cauda segments of epididymis and vas deferens of adult rhesus monkey and expressed as units per mg DNA. These enzymes were also estimated in epididymal segments and vas deferens of castrated and castrated-androgen replaced monkeys as well. Results indicated higher activities of most of the enzymes in vas deferens as compared to epididymal segments. All the enzymes showed marked reduction in epididymis and vas deferens after castration, the effect being much more pronounced in the epididymis, than in the vas. Androgen replacement in castrated monkeys stimulated most of the enzymes markedly in epididymis and in the vas deferens as compared to their castrated values. The response of cauda and vas deferens to exogenous androgen treatment was however moderate, as compared to the other epididymal segments. The studies indicate that energy metabolism in the epididymis (as well as in the vas deferens) is strictly androgen dependent and the energy charge of these target organs is likely to fall appreciably after castration, which may in turn affect many energy dependent processes of these organs (e.g. absorption, secretion of specific substances etc.) which have been considered important for sperm maturation and survival.
在成年恒河猴附睾和输精管的起始段、头部、体部和尾部，对三羧酸循环的九种酶的活性进行了评估，并以每毫克DNA的单位数表示。在去势猴以及去势并雄激素替代的猴的附睾段和输精管中也对这些酶进行了评估。结果表明，与附睾段相比，输精管中大多数酶的活性更高。去势后，附睾和输精管中所有酶的活性均显著降低，这种影响在附睾中比在输精管中更为明显。与去势后的数值相比，去势猴的雄激素替代显著刺激了附睾和输精管中的大多数酶。然而，与附睾的其他段相比，尾部和输精管对外源性雄激素治疗的反应较为适度。这些研究表明，附睾（以及输精管）中的能量代谢严格依赖雄激素，去势后这些靶器官的能量电荷可能会明显下降，这反过来可能会影响这些器官的许多能量依赖过程（例如特定物质的吸收、分泌等），而这些过程被认为对精子成熟和存活很重要。
