Afshan A, Jehangir M, Ashraf M, Waqar A, Chiotellis E
Nuclear Chemistry Division, Pakistan Institute of Nuclear Science and Technology, Islamabad.
Eur J Nucl Med. 1994 Sep;21(9):991-5. doi: 10.1007/BF00238126.
Ethyl cysteinate dimer (ECD) labelled with reduced technetium-99m has recently been proposed as a promising radiopharmaceutical for brain perfusion imaging. In the present study a single-component kit formulation has been developed, thus simplifying the radiolabelling procedure. A method of analysis by electrophoresis has also been developed, permitting identification of radiochemical impurities in the preparation. 99mTc-ECD prepared by the single-component kit was further evaluated in primates and humans. The results demonstrated that the complex is stable in vivo, rapidly extracted and retained in the brain tissue for a sufficient time for single-photon emission tomography studies. Therefore the present single-component kit formulation can be proposed as a reliable instant freeze-dried kit for routine preparation of 99mTc-ECD required for scintigraphic assessment of regional cerebral blood flow.
最近，用还原态锝-99m标记的半胱氨酸乙酯二聚体（ECD）被提议作为一种有前景的用于脑灌注显像的放射性药物。在本研究中，已开发出一种单组分试剂盒配方，从而简化了放射性标记程序。还开发了一种电泳分析方法，可用于鉴定制剂中的放射化学杂质。通过单组分试剂盒制备的99mTc-ECD在灵长类动物和人类中进行了进一步评估。结果表明，该复合物在体内稳定，能迅速被提取并在脑组织中保留足够长的时间用于单光子发射断层扫描研究。因此，目前的单组分试剂盒配方可被提议作为一种可靠的即时冻干试剂盒，用于常规制备闪烁显像评估局部脑血流所需的99mTc-ECD。
