King A, Smith P, Heath D
Department of Pathology, University of Liverpool, England.
Exp Mol Pathol. 1994 Aug;61(1):24-35. doi: 10.1006/exmp.1994.1023.
A group of Wistar rats was treated with two subcutaneous injections of monocrotaline, at 4 and 6 weeks of age. They were then killed 1 month after the initial injection. A second group of rats was born and reared in hypobaric hypoxia for 1 month before being killed. Both groups had age-matched controls. The ultrastructure of pulmonary arterioles from all groups was studied, and quantitative measurements were made of the volume densities of organelles within the cytoplasm of arteriolar smooth muscle cells. The pulmonary arterioles of rats treated with monocrotaline contained immature smooth muscle cells with coarse peripheral myofilaments and were bounded by thin indistinct elastic laminae. In contrast, the arteriolar smooth muscle cells of hypoxic rats were mature with fine myofilaments and bounded by electron dense laminae. When compared with both their respective controls and the alternative test group, the muscle cells from rats treated with monocrotaline had significantly lower volume densities of dense bodies, and the hypoxic muscle cells had significantly higher densities of mitochondria. The pulmonary arteriolar muscularization in rats would appear to be a nonuniform process producing smooth muscle cells with differing cytoplasmic features that suggest differing cellular functions.
一组Wistar大鼠在4周龄和6周龄时皮下注射两次野百合碱。然后在初次注射后1个月处死。第二组大鼠在低压低氧环境中出生并饲养1个月后处死。两组均有年龄匹配的对照组。研究了所有组肺小动脉的超微结构，并对小动脉平滑肌细胞质内细胞器的体积密度进行了定量测量。用野百合碱处理的大鼠肺小动脉含有不成熟的平滑肌细胞，其外周肌丝粗大，周围有薄而不清晰的弹性膜。相比之下，低氧大鼠的小动脉平滑肌细胞成熟，肌丝纤细，周围有电子致密膜。与各自的对照组和另一试验组相比，用野百合碱处理的大鼠的肌肉细胞致密体的体积密度显著降低，而低氧肌肉细胞的线粒体密度显著升高。大鼠肺小动脉肌化似乎是一个不均匀的过程，产生具有不同细胞质特征的平滑肌细胞，提示细胞功能不同。
