III组柑橘类病毒的核苷酸序列和结构特征

Nucleotide sequence and structural features of the group III citrus viroids.

作者信息

Rakowski A G, Szychowski J A, Avena Z S, Semancik J S

机构信息

Department of Plant Pathology, University of California, Riverside 92521.

出版信息

J Gen Virol. 1994 Dec;75 ( Pt 12):3581-4. doi: 10.1099/0022-1317-75-12-3581.

DOI:10.1099/0022-1317-75-12-3581

PMID:7996150

Abstract

The nucleotide sequence and secondary structure of two representative variants from the Group III citrus viroids. CVd-IIIa (297 bases) and CVd-IIIb (294 bases) were determined. The variants are related to the apple scar skin viroid (ASSVd) family. Although smaller in size than any of the ASSVd-related viroids, the central conserved region as well as most of the terminal conserved region of ASSVd is retained. The rod-like structural configuration (characteristic of ASSVd) of the variants as predicted by minimum free energy analysis is presented.

摘要

测定了III组柑橘类病毒中两个代表性变体CVd-IIIa（297个碱基）和CVd-IIIb（294个碱基）的核苷酸序列和二级结构。这些变体与苹果锈果类病毒（ASSVd）家族相关。尽管其大小比任何与ASSVd相关的类病毒都小，但仍保留了ASSVd的中央保守区域以及大部分末端保守区域。通过最小自由能分析预测了这些变体的棒状结构构型（ASSVd的特征）。