Di Serio F, Aparicio F, Alioto D, Ragozzino A, Flores R
Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia, Spain.
J Gen Virol. 1996 Nov;77 ( Pt 11):2833-7. doi: 10.1099/0022-1317-77-11-2833.
A new viroid associated with an apple fruit disorder similar to, but more severe than, the dapple apple disease induced in some varieties by apple scar skin viroid (ASSVd) has been found. The new viroid, tentatively termed apple dimple fruit viroid (ADFVd), is a circular RNA of 306 nucleotides which adopts a quasi-rod-like conformation of minimum free energy. It contains the core nucleotides of the central conserved region (CCR) of the ASSVd group as well as the terminal conserved region (TCR) present in all members of the ASSVd and potato spindle tuber viroid (PSTVd) monophyletic groups. ADFVd has the highest sequence similarity with ASSVd and the 294 nucleotide citrus viroid CVd-IIIb sharing with the latter an almost identical left terminal domain. The right- and left-hand termini of ADFVd are formed by almost perfect duplications of sequences found in the CCR upper and lower strands, respectively, of PSTVd and closely related viroids.
已发现一种与苹果果实病害相关的新类病毒，该病害类似于某些品种因苹果锈果类病毒（ASSVd）引发的花脸苹果病，但更为严重。这种新类病毒暂称为苹果凹果类病毒（ADFVd），是一种由306个核苷酸组成的环状RNA，其采用具有最小自由能的准棒状构象。它包含ASSVd组中央保守区（CCR）的核心核苷酸，以及ASSVd和马铃薯纺锤块茎类病毒（PSTVd）单系群所有成员中都存在的末端保守区（TCR）。ADFVd与ASSVd具有最高的序列相似性，并且与294个核苷酸的柑橘类病毒CVd-IIIb共享几乎相同的左末端结构域。ADFVd的右手和左手末端分别由PSTVd和密切相关类病毒CCR上、下链中发现的序列几乎完美重复形成。
