Champelovier P, Valiron O, Michèle J, Dominique L, Scigncurin D
Laboratoire de Cytologie, Centre Hospitalier Universitaire de Grenoble, France.
Leuk Res. 1994 Dec;18(12):903-18. doi: 10.1016/0145-2126(94)90102-3.
The human leukemic cell line LAMA-84 was established and characterized as an erythromegakaryocytic cell line. In the present study we show that these cells can differentiate in estrone-treated athymic mice and give rise to an erythroeosinophilic cell line (LAMA-87). This new cell line expressed glycoporin A, alpha beta and gamma globin chain mRNA but also eosinophilic peroxidase. Hemin slightly increased the total hemoglobin production of the cells and phorbol diester (TPA), dimethyl sulfoxide (DMSO) and sodium butyrate (SB) increased the expression of megakaryocytic markers (gpIIb/IIIa complex). When inoculated into non-treated athymic mice, LAMA-87 cells can differentiate to give rise to eosinomonocytic cells (LAMA-88). This new cell line expresses eosinophilic peroxidase, Luxol fast blue stain and synthesizes lysozyme. Depending on the inducer used, LAMA-88 can differentiate along a monocytic lineage (TPA, DMSO, SB and vitamin D3). These three LAMA cell lines should be useful in further studies of the molecular regulation of the pluripotent cell commitment and may provide a model for the understanding of human hematopoiesis.
人白血病细胞系LAMA - 84被建立并鉴定为红巨核细胞系。在本研究中，我们表明这些细胞可在经雌酮处理的无胸腺小鼠中分化，并产生一种红嗜酸性细胞系（LAMA - 87）。这个新细胞系表达血型糖蛋白A、αβ和γ珠蛋白链mRNA，还表达嗜酸性过氧化物酶。氯化血红素略微增加了细胞的总血红蛋白产量，佛波酯（TPA）、二甲基亚砜（DMSO）和丁酸钠（SB）增加了巨核细胞标志物（糖蛋白IIb/IIIa复合物）的表达。当接种到未处理的无胸腺小鼠中时，LAMA - 87细胞可分化产生嗜酸性单核细胞（LAMA - 88）。这个新细胞系表达嗜酸性过氧化物酶、髓磷脂碱性蛋白染色并合成溶菌酶。根据所使用的诱导剂不同，LAMA - 88可沿单核细胞谱系分化（TPA、DMSO、SB和维生素D3）。这三种LAMA细胞系应有助于进一步研究多能细胞定向分化的分子调控，并可能为理解人类造血作用提供一个模型。
