Blom T, Nilsson G, Sundström C, Nilsson K, Hellman L
Department of Medical Immunology, University of Uppsala, Sweden.
Scand J Immunol. 1996 Jul;44(1):54-61. doi: 10.1046/j.1365-3083.1996.d01-84.x.
LAMA-84, a human leucocytic cell line, which upon establishment was described as having megakaryocytic, erythroid and granulocytic characteristics, was analysed for expression of various differentiation markers. In addition to some of the previously described phenotypic characteristics, this cell line was found to express mRNA for several proteins characteristic for basophilic leucocytes and mast cells. The authors show that LAMA-84 cells express mRNA for the mast cell tryptase, the proteoglycan core protein, carboxypeptidase A and the alpha and beta chains of the high affinity IgE receptor (Fc epsilon RI). The authors examined the potential of LAMA-84 to differentiate in serum-free medium or after DMSO or PMA treatment. Depending on the inducing factor, surface expression of the Fc epsilon RI alpha-chain was increased from 20% to 35-50% of the cells and mRNA levels for tryptase were increased in serum-free medium and after DMSO treatment. LAMA-84 was found to express CD13, CDw17, CD29, CD33, CD40, CD45 and CD117. Furthermore, mRNA for the eosinophil/basophil markers Charcot-Leyden crystal (CLC) protein and the major basic protein (MBP), as well as the erythrocyte differentiation marker alpha-globin, was detected. However, the authors observed only trace amounts of mRNA for another erythroid differentiation marker (glycophorin), trace amounts of the megakaryocytic marker GPIIIa, and no detectable level of GPIb alpha. By comparing the expression pattern of a panel of differentiation markers in LAMA-84, and a second human cell line (KU812) expressing a basophil phenotype, it is evident that these cell lines, which presently are the only two cell lines identified with basophilic characteristics, share a large number of phenotypic characteristics.
LAMA-84是一种人白细胞系，建立时被描述为具有巨核细胞、红系和粒系特征，对其各种分化标志物的表达进行了分析。除了一些先前描述的表型特征外，该细胞系还被发现表达几种嗜碱性白细胞和肥大细胞特有的蛋白质的mRNA。作者表明，LAMA-84细胞表达肥大细胞类胰蛋白酶、蛋白聚糖核心蛋白、羧肽酶A以及高亲和力IgE受体（FcεRI）的α和β链的mRNA。作者研究了LAMA-84在无血清培养基中或经二甲基亚砜（DMSO）或佛波酯（PMA）处理后分化的潜力。根据诱导因子的不同，FcεRIα链的表面表达在细胞中的比例从20%增加到35% - 50%，类胰蛋白酶的mRNA水平在无血清培养基中和DMSO处理后增加。发现LAMA-84表达CD13、CDw17、CD29、CD33、CD40、CD45和CD117。此外，还检测到嗜酸性粒细胞/嗜碱性粒细胞标志物夏科-莱登结晶（CLC）蛋白和主要碱性蛋白（MBP）以及红细胞分化标志物α-珠蛋白的mRNA。然而，作者仅观察到另一种红系分化标志物（血型糖蛋白）的微量mRNA、巨核细胞标志物GPIIIa的微量mRNA，且未检测到GPIbα的表达水平。通过比较LAMA-84和另一种表达嗜碱性表型的人细胞系（KU812）中一组分化标志物的表达模式，很明显，这两种目前仅有的被鉴定具有嗜碱性特征的细胞系具有大量共同的表型特征。
