Sobieszczańska B M, Grzybek-Hryncewicz K
Katedra i Zakład Mikrobiologii AM we Wrocławiu.
Med Dosw Mikrobiol. 1994;46(3):175-9.
This investigation was aimed at selection of optimal serological test for diagnosis of borreliosis occurring at our territory. In our investigations, and indirect immunofluorescence test (IF) was used in which antigen consisted of an American strain of Borrelia burgdorferi B31 and immunoenzymatic test (IE) with an antigen prepared from an European strain. Hundred sixty eight sera were tested, including 138 sera received from patients with suspection of borreliosis or directed for testing because of suspected tick bite. Thirty sera from healthy blood donors served as a control. In 30 out of 138 patients with suspected borreliosis presence of specific antibodies in IF or IE test was detected. In control group positive results were obtained in 4 persons. From our study comparing two tests occurs that more suitable for serodiagnosis of borreliosis on our territory is determination employing as an antigen the European strain of Borrelia burgdorferi.
本研究旨在选择用于诊断我国境内莱姆病的最佳血清学检测方法。在我们的研究中，使用了间接免疫荧光试验（IF），其抗原为由美国伯氏疏螺旋体B31菌株组成，以及免疫酶试验（IE），其抗原由欧洲菌株制备。检测了168份血清，其中包括138份来自疑似莱姆病患者或因疑似蜱叮咬而送检的血清。30份来自健康献血者的血清作为对照。在138例疑似莱姆病患者中，有30例在IF或IE试验中检测到特异性抗体。在对照组中，有4人获得阳性结果。从我们比较两种检测方法的研究中可以看出，在我国境内，更适合莱姆病血清学诊断的方法是采用欧洲伯氏疏螺旋体菌株作为抗原进行检测。
