Fernandes A J, Vitor R W, Dias J C
Posto Avançado de Estudos Emmanuel Dias, Laboratório de Biologia de Triatomineos e Epidemiologia da doença de Chagas, Centro de Pesquisas René Rachou, Belo Horizonte, MG, Brasil.
Rev Inst Med Trop Sao Paulo. 1994 Jan-Feb;36(1):11-7.
The experimental infection by Trypanosoma cruzi was studied in nine young goats. These animals were inoculated by intraperitoneal route with 10(3) trypomastigotes/Kg body weight with strains 147 (Group 1) and 229 (Group 2), isolated from chronic Chagasic patients proceeding from Bambuí, Minas Gerais, Brazil. The infected animals were submitted to fresh blood examinations, xenodiagnosis, hemoculture and serology (IFAT and ELISA). The follow up of T. cruzi infection ranged from 7 to 30 months and it was possible to note a markable difference concerning the course of infection between the two groups. The parasite was visualized, for the first time in experimentally infected goats in the acute period through fresh blood examinations and xenodiagnosis and through hemoculture and xenodiagnosis during the chronic period of the Chagasic infection. These results suggest that, under some epidemiological conditions, goats may be important in the sylvatic and peridomiciliar transmission cycles of T. cruzi specially young animals.
在九只幼山羊身上研究了克氏锥虫的实验性感染。这些动物通过腹腔途径接种了每千克体重10³个锥鞭毛体,菌株为147(第1组)和229(第2组),这些菌株是从巴西米纳斯吉拉斯州班布伊的慢性恰加斯病患者中分离出来的。对感染动物进行了新鲜血液检查、异种接种诊断、血液培养和血清学检测(间接荧光抗体试验和酶联免疫吸附测定)。克氏锥虫感染的随访时间为7至30个月,并且可以注意到两组之间在感染过程方面存在显著差异。在急性期,通过新鲜血液检查和异种接种诊断首次在实验感染的山羊中观察到寄生虫;在恰加斯病感染的慢性期,通过血液培养和异种接种诊断观察到寄生虫。这些结果表明,在某些流行病学条件下,山羊可能在克氏锥虫的野生和家栖传播循环中起重要作用,特别是幼龄动物。