Lissaldo A M, Hoshino-Shimizu S, Umezawa E S, Stolf A M
Instituto de Ciências Biomédicas, USP, São Paulo, Brasil.
Rev Inst Med Trop Sao Paulo. 1994 Mar-Apr;36(2):163-6. doi: 10.1590/s0036-46651994000200012.
The alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) was assessed in dot-ELISA for the diagnosis of Chagas' disease. Serum samples (355) from chagasic and non-chagasic patients were studied, and IgG antibodies to ASEA were found in all patients with chronic Chagas' disease. In non-chagasic patients 95.6% were negative, except for those with leishmaniasis (visceral and mucocutaneous), and some patients from control group reacted in low titers. The data indicate that dot-ELISA using ASEA is suitable for seroepidemiologic surveys to be employed in endemic areas for Chagas' disease.
