Sweeney J D, Holme S, Heaton W A, Campbell D, Bowen M L
American Red Cross Blood Services, Mid-Atlantic Region and Eastern Virginia Medical School, Norfolk.
Transfusion. 1995 Jan;35(1):46-9. doi: 10.1046/j.1537-2995.1995.35195090660.x.
EDTA pseudothrombocytopenia (PTCP) is an in vitro artifact in which the anticoagulation of blood with EDTA is associated with in vitro agglutination of platelets, resulting in a spuriously low platelet count. In apheresis donors, whole-blood samples for complete blood counts are routinely drawn into tubes anti-coagulated with EDTA.
Records of apheresis donors were examined to identify persons in whom the postdonation counts were less than 100 x 10(9) per L. Identified donors were studied to confirm the presence of PTCP by drawing blood samples into EDTA, heparin, and trisodium citrate for serial platelet counts at room-temperature incubation. Platelet counts in citrated plasma were measured before and after the addition of EDTA. A single HLA-matched component from an identified PTCP donor was monitored for response by corrected count increment in the recipient.
A total of nine donations were identified, involving 2 donors from a population of 945 donors (prevalence 0.2%). On testing, both donors were confirmed to have PTCP. The addition of EDTA to citrated plasma did not affect the platelet count. Response in a recipient to an HLA-matched component showed an acceptable corrected count increment.
PTCP may occur in plateletpheresis donors and result in needless medical referral or donor deferral. PTCP does not appear to alter the yield content of the component or to be passively transferred to a recipient.
乙二胺四乙酸（EDTA）假性血小板减少症（PTCP）是一种体外假象，其中用EDTA抗凝血与血小板的体外凝集有关，导致血小板计数假性降低。在单采献血者中，用于全血细胞计数的全血样本通常被采集到用EDTA抗凝的试管中。
检查单采献血者的记录，以识别献血后计数低于每升100×10⁹的人员。对识别出的献血者进行研究，通过将血样采集到EDTA、肝素和枸橼酸钠中，在室温孵育下进行系列血小板计数，以确认PTCP的存在。在添加EDTA之前和之后测量枸橼酸血浆中的血小板计数。通过受者的校正计数增加值监测来自一名已识别的PTCP献血者的单个HLA匹配成分的反应。
共识别出9次献血，涉及945名献血者群体中的2名献血者（患病率0.2%）。经检测，两名献血者均被确诊为PTCP。向枸橼酸血浆中添加EDTA不影响血小板计数。受者对HLA匹配成分的反应显示出可接受的校正计数增加值。
PTCP可能发生在血小板单采献血者中，并导致不必要的医学转诊或献血者延期。PTCP似乎不会改变成分的产量，也不会被动转移给受者。
