[用于检测新生儿尿液中巨细胞病毒的聚合酶链反应优化]
[The optimization of the PCR for detecting cytomegalovirus in the urine of newborn infants].
作者信息
Vinogradskaia G R, Novikova L N, Bashmakova M A
出版信息
Vopr Virusol. 1994 Jul-Aug;39(4):171-4.
PMID:7998396
Abstract
A quantitative comparison of 4-primer PCR-diagnosticum potentials for detection of cytomegalovirus DNA in urine specimens in different methods for DNA isolation was carried out. Comparatively simple DNA isolation procedures were considered for express diagnosis in clinical conditions. A scheme of successive use of diagnostic preparations was proposed.
摘要
对不同DNA分离方法检测尿标本中巨细胞病毒DNA的4引物PCR诊断潜力进行了定量比较。考虑采用相对简单的DNA分离程序用于临床条件下的快速诊断。提出了诊断制剂连续使用方案。
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