用于检测人巨细胞病毒DNA的非放射性聚合酶链反应-酶联免疫吸附测定法
Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA.
作者信息
Allen R D, Pellett P E, Stewart J A, Koopmans M
机构信息
Viral Exanthems and Herpesvirus Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
出版信息
J Clin Microbiol. 1995 Mar;33(3):725-8. doi: 10.1128/jcm.33.3.725-728.1995.
Abstract
We developed a rapid, sensitive, and specific PCR-based assay for human cytomegalovirus (HCMV). The assay includes primer and probe sequences derived from conserved HCMV nucleotide sequences and nonradioactive hybridization-confirmation. The assay detected between 10 and 100 viral genomes. All HCMV clinical isolates tested (39 of 39) gave positive reactions.
摘要
我们开发了一种针对人巨细胞病毒（HCMV）的基于PCR的快速、灵敏且特异的检测方法。该检测方法包括源自保守的HCMV核苷酸序列的引物和探针序列以及非放射性杂交确认。该检测方法能检测出10至100个病毒基因组。所有检测的HCMV临床分离株（39株中的39株）均呈阳性反应。
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本文引用的文献
1
Detection of adenovirus in clinical specimens by polymerase chain reaction and liquid-phase hybridization quantitated by time-resolved fluorometry.通过聚合酶链反应及时间分辨荧光法定量液相杂交检测临床标本中的腺病毒。
J Clin Microbiol. 1993 Jul;31(7):1886-91. doi: 10.1128/jcm.31.7.1886-1891.1993.
2
Polymerase chain reaction to detect cytomegalovirus DNA in the cerebrospinal fluid of neonates with congenital infection.聚合酶链反应检测先天性感染新生儿脑脊液中的巨细胞病毒DNA。
J Infect Dis. 1994 Jun;169(6):1334-7. doi: 10.1093/infdis/169.6.1334.
3
Detection of cytomegalovirus in urine from newborns by using polymerase chain reaction DNA amplification.利用聚合酶链反应DNA扩增技术检测新生儿尿液中的巨细胞病毒。
J Infect Dis. 1988 Dec;158(6):1177-84. doi: 10.1093/infdis/158.6.1177.
4
Enzymatic amplification of human cytomegalovirus sequences by polymerase chain reaction.通过聚合酶链反应对人巨细胞病毒序列进行酶促扩增。
J Clin Microbiol. 1989 Aug;27(8):1802-9. doi: 10.1128/jcm.27.8.1802-1809.1989.
5
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Med J Aust. 1991 Mar 18;154(6):383-5. doi: 10.5694/j.1326-5377.1991.tb121127.x.
7
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9
Molecular epidemiology of envelope glycoprotein H of human cytomegalovirus.
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Virology. 1992 May;188(1):388-90. doi: 10.1016/0042-6822(92)90771-g.
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