Yu S, Aaskov J G
World Health Organization Collaborative Centre for Arbovirus Reference and Research, School of Life Science, Queensland University of Technology, Brisbane, Australia.
Vaccine. 1994 Sep;12(12):1118-24. doi: 10.1016/0264-410x(94)90182-1.
Ross River virus is a mosquito-borne alphavirus which causes several thousand cases of arthritis (epidemic polyarthritis) each year. In this study, binary ethylenimine (BEI) was used to destroy the infectivity of this virus without abolishing the antigenicity or immunogenicity of the virion. Mice immunized intramuscularly with BEI-inactivated virus, with or without Alhydrogel adjuvant, produced antibody which neutralized Ross River virus in vitro, and the mice also failed to develop viraemia when challenged intravenously with live virus. Serum neutralization and in vivo protection were greatest when BEI-inactivated virus was administered without adjuvant.
罗斯河病毒是一种由蚊子传播的甲病毒，每年会导致数千例关节炎（流行性多关节炎）病例。在本研究中，使用双乙基亚胺（BEI）来破坏该病毒的传染性，同时不消除病毒粒子的抗原性或免疫原性。用BEI灭活病毒肌肉注射免疫的小鼠，无论有无氢氧化铝佐剂，均产生了能在体外中和罗斯河病毒的抗体，并且当用活病毒静脉攻击时，这些小鼠也未出现病毒血症。当不使用佐剂给予BEI灭活病毒时，血清中和作用和体内保护作用最强。
