Aaskov J, Williams L, Yu S
WHO Collaborating Centre for Arbovirus Reference and Research, School of Life Science, Queensland University of Technology, Brisbane, Australia.
Vaccine. 1997 Aug-Sep;15(12-13):1396-404. doi: 10.1016/s0264-410x(97)00051-0.
A killed Ross River virus vaccine is being developed in an effort to prevent the, ca 5000 cases of epidemic polyarthritis which occur in Australia each year. The symptoms of epidemic polyarthritis commonly last 30-40 weeks and 25% of patients have symptoms for a year or more. There is no cure. Although there was some strain to strain variation, particularly after a single injection, outbred and inbred strains of mice all produced significant levels of anti-Ross River virus antibody after intramuscular (i.m.) injection with 24 h BEI inactivated, sucrose gradient purified, Ross River virus vaccine. Mice immunized i.m. with two 20 micrograms doses of vaccine or live virus produced similar levels of neutralizing antibody but the reaction of IgG 2a and IgG 2b antibody from these two groups of mice to Ross River virus proteins in western blots differed. Antibody from BALB/c mice immunized with this vaccine neutralized all strains of Ross River virus tested, in vitro, albeit to different degrees.
一种用于预防罗斯河病毒的灭活疫苗正在研发中，澳大利亚每年约有5000例流行性多关节炎病例。流行性多关节炎的症状通常持续30至40周，25%的患者症状会持续一年或更长时间。目前尚无治愈方法。尽管不同毒株之间存在一些差异，尤其是单次注射后，但在用24小时BEI灭活、蔗糖梯度纯化的罗斯河病毒疫苗进行肌肉注射后，远交系和近交系小鼠均产生了显著水平的抗罗斯河病毒抗体。用两剂20微克疫苗或活病毒进行肌肉注射免疫的小鼠产生了相似水平的中和抗体，但这两组小鼠的IgG 2a和IgG 2b抗体在蛋白质印迹法中对罗斯河病毒蛋白的反应有所不同。用该疫苗免疫的BALB/c小鼠产生的抗体在体外能中和所有测试的罗斯河病毒毒株，尽管程度不同。
