Momburg F, Roelse J, Neefjes J, Hämmerling G J
Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Behring Inst Mitt. 1994 Jul(94):26-36.
Our knowledge about intracellular pathways involved in the presentation of antigens was considerably broadened with the recent discovery of peptide transporters encoded in the major histocompatibility complex. The transporter associated with antigen processing (TAP) belongs to an evolutionary conserved family of multimembrane-spanning translocators that bind ATP and show specificity for a variety of different substrates. TAP mediates the translocation of peptides, generated by cytosolic degradation of protein antigens, into the lumen of the endoplasmic reticulum where they bind to newly synthesized MHC class I molecules. A novel assay has been employed to elucidate the details of TAP-mediated peptide transport. The results indicate that TAP selects peptides of sequence and length according to the requirements of MHC class I molecules in different species.
随着近期在主要组织相容性复合体中编码的肽转运体的发现，我们关于参与抗原呈递的细胞内途径的知识得到了极大的拓展。与抗原加工相关的转运体（TAP）属于一个进化上保守的多跨膜转运体家族，该家族结合ATP并对多种不同底物具有特异性。TAP介导由蛋白质抗原的胞质降解产生的肽转运到内质网腔中，在那里它们与新合成的MHC I类分子结合。一种新的检测方法已被用于阐明TAP介导的肽转运的细节。结果表明，TAP根据不同物种中MHC I类分子的需求选择序列和长度合适的肽。
