Sørensen E S, Rasmussen L K, Møller L, Jensen P H, Højrup P, Petersen T E
Protein Chemistry Laboratory, University of Aarhus, Denmark.
Biochem J. 1994 Nov 15;304 ( Pt 1)(Pt 1):13-6. doi: 10.1042/bj3040013.
Here we report the identification of two transglutaminase-reactive glutamines (Gln-34 and Gln-36) in bovine osteopontin (OPN). Sequence alignment revealed that these glutamines are conserved in all known OPN sequences, indicating a functional importance of this region of the protein. Furthermore, immunological analysis of bovine bone demonstrated that OPN is present in high-molecular-mass complexes in vivo. These findings support the functional aspects of a transglutaminase-catalysed cross-linking of OPN in facilitating cellular attachment and tissue calcification.
在此，我们报告在牛骨桥蛋白（OPN）中鉴定出两种转谷氨酰胺酶反应性谷氨酰胺（Gln-34和Gln-36）。序列比对显示，这些谷氨酰胺在所有已知的OPN序列中都是保守的，表明该蛋白区域具有功能重要性。此外，对牛骨的免疫学分析表明，OPN在体内以高分子量复合物形式存在。这些发现支持了转谷氨酰胺酶催化的OPN交联在促进细胞黏附和组织钙化方面的功能作用。
