Lindh I, Griffiths W J, Bergman T, Sjövall J
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Rapid Commun Mass Spectrom. 1994 Oct;8(10):797-803. doi: 10.1002/rcm.1290081002.
A series of small peptides has been studied by negative-ion fast-atom bombardment mass spectrometry with collision-induced dissociation. It has been found that by derivatizing peptides with 4-aminonaphthalenesulphonic acid in a peptide linkage at the C-terminus, negative-ion formation can be enhanced and fragmentation in collision-induced dissociation reactions controlled. The peptide-naphthalenesulphonates show charge-remote fragmentations and the resultant spectra give sequence information.
利用负离子快原子轰击质谱法并结合碰撞诱导解离对一系列小肽进行了研究。研究发现，通过在肽链的C端肽键处用4-氨基萘磺酸对肽进行衍生化，可以增强负离子的形成，并控制碰撞诱导解离反应中的碎片化过程。肽-萘磺酸盐显示出电荷远程碎片化，所得光谱给出了序列信息。
