Benchimol M
Laboratório de Ultraestrutura Celular Hertha Meyer, Universidade Federal do Rio de Janeiro, Brazil.
Microsc Res Tech. 1994 Sep 1;29(1):37-46. doi: 10.1002/jemt.1070290106.
Tritrichomonas foetus was studied using different physical and chemical fixation methods such as fast-freezing (by high pressure, "slam-freezing," and jet-propane), freeze-substitution, conventional freeze-fracture and deep-etching, cryoultramicrotomy, and routine preparation for transmission electron microscopy. The use of fast-freezing fixation (FFF) proved to be superior in terms of structural preservation due to the rapidity of this fixation compared to that obtained using conventional chemical fixation. The low temperature techniques used here were useful to confirm data already obtained by conventional freeze-fracture using chemical fixation and cryoprotection, such as the presence of flagellar rosettes and costa structure. Cryoultramicrotomy and slam-freezing also demonstrated the presence of hair-like structures projecting out from the protozoan surface. New aspects of organelles of T. foetus were demonstrated.
利用不同的物理和化学固定方法对胎儿三毛滴虫进行了研究，这些方法包括快速冷冻（通过高压、“冲击冷冻”和喷射丙烷）、冷冻置换、传统的冷冻断裂和深度蚀刻、低温超薄切片术以及透射电子显微镜的常规制备。与传统化学固定相比，快速冷冻固定（FFF）由于其固定速度快，在结构保存方面被证明更具优势。这里使用的低温技术有助于证实通过使用化学固定和冷冻保护的传统冷冻断裂已经获得的数据，如鞭毛玫瑰花结和肋结构的存在。低温超薄切片术和冲击冷冻也证明了原生动物表面伸出毛发状结构。揭示了胎儿三毛滴虫细胞器的新特征。
