Terada N, Ohno S
Department of Anatomy, Yamanashi Medical University, Japan.
Kaibogaku Zasshi. 1998 Dec;73(6):587-93.
This article summarizes our research on the morphology of dynamically changed erythrocyte shapes, as revealed by electron microscopy with cryofixation techniques. Cryofixation is a better way to immobilize functioning morphology in cells and tissues than conventional fixation methods because of faster freezing time. Our cryofixation techniques were used to detect erythrocyte shapes in flow and ultrastructural changes of membrane skeletal components. This required that we develop some new preparation techniques which we called, erythrocyte-splitting method, in vivo cryotechnique or in vitro cryotechnique for erythrocytes. By using various combinations of these cryofixation techniques with freeze-fracturing, freeze-substitution or deep-etching method, the native morphology of erythrocytes under various physiological or pathological conditions could be assessed by scanning or transmission electron microscopy.
本文总结了我们通过冷冻固定技术的电子显微镜所揭示的动态变化红细胞形态的研究。由于冷冻时间更快，与传统固定方法相比，冷冻固定是一种更好地固定细胞和组织中功能形态的方法。我们的冷冻固定技术用于检测流动中的红细胞形态以及膜骨架成分的超微结构变化。这就要求我们开发一些新的制备技术，我们称之为红细胞裂解方法、体内冷冻技术或红细胞体外冷冻技术。通过将这些冷冻固定技术与冷冻断裂、冷冻置换或深度蚀刻方法进行各种组合，可以通过扫描或透射电子显微镜评估各种生理或病理条件下红细胞的天然形态。
