脂磷蛋白作为鲎（美洲鲎）血淋巴中的脂质载体。

Lipophorin as a lipid carrier in the hemolymph of the horseshoe crab, Limulus polyphemus.

作者信息

Rehn K G, Lee R F

机构信息

Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Brasil.

出版信息

Braz J Med Biol Res. 1994 May;27(5):1135-9.

PMID:8000334

Abstract

A high-density lipoprotein (HDL) was obtained from the hemolymph of Limulus polyphemus in yields generally less than 30 micrograms/ml by ultracentrifugal flotation. SDS-PAGE revealed two apolipoproteins with masses similar to those of apolipophorins (apoLp-I, 265 +/- 14 kDa; apoLp-II, 89 +/- 6 kDa). Lipid composition was different from both insect lipophorin and crustacean HDL, and showed less diacylglycerols than triacylglycerols (3.8% and 36.2% of total lipids, respectively). Since Limulus polyphemus is closely related to precambrian chelicerates, our results confirm that lipophorin was present early in the evolution of arthropods.

摘要

通过超速离心浮选法，从鲎的血淋巴中获得了高密度脂蛋白（HDL），产量通常低于30微克/毫升。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）显示出两种载脂蛋白，其质量与载脂蛋白（载脂蛋白-I，265±14 kDa；载脂蛋白-II，89±6 kDa）相似。脂质组成不同于昆虫的脂转运蛋白和甲壳类动物的HDL，并且二酰甘油比三酰甘油少（分别占总脂质的3.8%和36.2%）。由于鲎与前寒武纪螯肢动物密切相关，我们的结果证实脂转运蛋白在节肢动物进化早期就已存在。