Robbs S L, Ryan R O, Schmidt J O, Keim P S, Law J H
J Lipid Res. 1985 Feb;26(2):241-7.
Most insects have a major lipoprotein species in the blood (hemolymph) that serves to transport fat from the midgut to the storage depots in fat body cells and from the fat body to peripheral tissues. The generic name lipophorin is used for this lipoprotein. In larvae of the honeybee, Apis mellifera, a lipophorin has been found with properties that correlate well with those of the only other lipophorin reported for an immature insect, that of the tobacco hornworm, Manduca sexta. The honeybee lipophorin (Mr = 530,000) has a density of 1.13 g/ml, contains approximately 41% lipid and 59% protein, and contains two apoproteins, apoLp-I, Mr = 250,000 and apoLp-II, Mr = 80,000, both of which are glycosylated. The lipids consist predominantly of polar lipids, of which phospholipids and diacylglycerols represent 60% of the total. When the intact lipophorin is treated with trypsin, apoLp-I is rapidly proteolyzed, while apoLp-II is resistant, indicating a difference in exposure of the two apoproteins to the aqueous environment. Honeybee apoLp-II cross-reacts with antibodies to M. sexta apoLp-II, but not to anti-M. sexta apoLp-I. No cross-reactivity of honeybee apoLp-I to anti-M. sexta apoLp-I was observed.
大多数昆虫血液（血淋巴）中有一种主要的脂蛋白，其作用是将脂肪从中肠运输到脂肪体细胞中的储存库，以及从脂肪体运输到外周组织。这种脂蛋白的通用名称是脂运载蛋白。在蜜蜂（Apis mellifera）幼虫中，已发现一种脂运载蛋白，其特性与已报道的另一种未成熟昆虫——烟草天蛾（Manduca sexta）的脂运载蛋白特性高度相关。蜜蜂脂运载蛋白（Mr = 530,000）的密度为1.13 g/ml，约含41%的脂质和59%的蛋白质，包含两种载脂蛋白，即载脂蛋白Lp-I（Mr = 250,000）和载脂蛋白Lp-II（Mr = 80,000），二者均为糖基化蛋白。脂质主要由极性脂质组成，其中磷脂和二酰基甘油占总量的60%。当完整的脂运载蛋白用胰蛋白酶处理时，载脂蛋白Lp-I迅速被蛋白水解，而载脂蛋白Lp-II具有抗性，这表明两种载脂蛋白暴露于水环境的情况存在差异。蜜蜂载脂蛋白Lp-II与烟草天蛾载脂蛋白Lp-II的抗体发生交叉反应，但与烟草天蛾载脂蛋白Lp-I的抗体不发生交叉反应。未观察到蜜蜂载脂蛋白Lp-I与烟草天蛾载脂蛋白Lp-I的抗体发生交叉反应。
