Pinkert C A, Galbreath E J, Yang C W, Striker L J
Department of Comparative Medicine, University of Alabama at Birmingham 35294-0019.
Transgenic Res. 1994 Nov;3(6):401-5. doi: 10.1007/BF01976771.
Transgenic pigs were created that harboured a phosphoenolpyruvate carboxykinase-bovine growth hormone construct (PEPCK-bGH). Four founder animals and two transgenic offspring from one line were evaluated between 6 1/2 and 12 months of age. There was no evidence of severe hepatic or renal lesions in these pigs, which characterised transgenic PEPCK-bGH mice previously described. While glomerular and tubular lesions in kidney sections were not identified in the transgenic pigs, mesangial cell proliferation was observed in two transgenic offspring from a single line. Additionally, glomerular size was significantly increased in four of four puberal transgenic swine when compared to age- and sex-matched controls (28.30 +/- 4.1 vs. 14.2 +/- 2.7 x 10(5) microns 3; representing 3 transgenic lines, p < 0.05). Surprisingly, no mature adipocytes were observed in subcutaneous sections obtained in transgenic GH pigs. Histological evaluation of these transgenic pigs further illustrates the requirement for precise control of growth-related genes and their protein products.
培育出了携带磷酸烯醇丙酮酸羧激酶 - 牛生长激素构建体（PEPCK - bGH）的转基因猪。对4只奠基动物和来自一个品系的2只转基因后代在6个半月至12个月龄之间进行了评估。这些猪没有出现之前所描述的转基因PEPCK - bGH小鼠所特有的严重肝脏或肾脏病变的迹象。虽然在转基因猪的肾脏切片中未发现肾小球和肾小管病变，但在来自单个品系的2只转基因后代中观察到了系膜细胞增殖。此外，与年龄和性别匹配的对照相比，4只青春期转基因猪中有4只的肾小球大小显著增加（28.30±4.1对14.2±2.7×10(5)微米3；代表3个转基因品系，p<0.05）。令人惊讶的是，在转基因生长激素猪的皮下切片中未观察到成熟脂肪细胞。对这些转基因猪的组织学评估进一步说明了对生长相关基因及其蛋白质产物进行精确控制的必要性。
