由显微注射金属硫蛋白-生长激素融合基因的卵发育而成的小鼠显著生长。
Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes.
作者信息
Palmiter R D, Brinster R L, Hammer R E, Trumbauer M E, Rosenfeld M G, Birnberg N C, Evans R M
出版信息
Nature. 1982 Dec 16;300(5893):611-5. doi: 10.1038/300611a0.
Abstract
A DNA fragment containing the promoter of the mouse metallothionein-I gene fused to the structural gene of rat growth hormone was microinjected into the pronuclei of fertilized mouse eggs. Of 21 mice that developed from these eggs, seven carried the fusion gene and six of these grew significantly larger than their littermates. Several of these transgenic mice had extraordinarily high levels of the fusion mRNA in their liver and growth hormone in their serum. This approach has implications for studying the biological effects of growth hormone, as a way to accelerate animal growth, as a model for gigantism, as a means of correcting genetic disease, and as a method of farming valuable gene products.
摘要
将一个含有与大鼠生长激素结构基因融合的小鼠金属硫蛋白-I基因启动子的DNA片段显微注射到受精小鼠卵的原核中。从这些卵发育而来的21只小鼠中，7只携带融合基因，其中6只比它们的同窝小鼠长得明显更大。这些转基因小鼠中有几只肝脏中的融合mRNA水平和血清中的生长激素水平异常高。这种方法对于研究生长激素的生物学效应、作为加速动物生长的一种方式、作为巨人症的模型、作为纠正遗传疾病的手段以及作为生产有价值基因产物的一种方法都有意义。
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