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[用于检测胰岛A细胞的海登海因苏木精铁染色法的改良]

[Modification of the Heidenhain's method of staining with iron hematoxylin for detection of the A-cells of the islands of Langerhans].

作者信息

Zharkov V P, Boĭko R T

出版信息

Probl Endokrinol (Mosk). 1978 Jul-Aug;24(4):68-9.

PMID:80006
Abstract

The authors suggest to use iron hematoxylin according to Haidenhain's prescription, with the subsequent passage of stained and differentiated histological sections through the ammonia solution for detection of A-cells in the pancreatic islets. The A-cells cytoplasm stained dark blue intensively; as to the nuclear structures--they acquired bluish-black tints. The method can be used for the study of morphology, topography and the number of A-cells in the islets.

摘要

作者建议按照海登海因的处方使用苏木精铁,随后将染色并分化的组织学切片通过氨溶液,以检测胰岛中的A细胞。A细胞的细胞质强烈染成深蓝色;至于核结构——它们呈现出蓝黑色调。该方法可用于研究胰岛中A细胞的形态、分布位置和数量。

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