Akmaev I G, Rabkina A E, Kabolova Z A
Probl Endokrinol (Mosk). 1976;22(2):80-3.
The authors describe a method of vital detection and isolation of individual islands of Langerhans in rats. This method is based on the capacity of the beta-cells of the islands to accumulate the stain during life. Since the stain (0.05% neutral red solution) is harmless and it is rapidly eliminated by the cells this method permits vital marking on the surface of the pancreas of individual islands and their distinguishment under steroscopic microscope; these islands can be isolated from the surrounding parenchyma of the pancreas with the aid of a microknife. Thus obtained islands of the pancreas could be used as material for experiments with implantation and for other experimental studies.
作者描述了一种在大鼠体内对胰岛进行活体检测和分离的方法。该方法基于胰岛β细胞在活体中积累染料的能力。由于该染料（0.05%中性红溶液）无害且能被细胞迅速清除，因此这种方法可对单个胰岛在胰腺表面进行活体标记，并在立体显微镜下将其区分出来；借助微型刀可将这些胰岛从胰腺周围实质中分离出来。如此获得的胰腺胰岛可作为植入实验及其他实验研究的材料。
