Bernacca G M, Tobasnick G, Wheatley D J
University Department of Cardiac Surgery, Royal Infirmary, Glasgow.
J Heart Valve Dis. 1994 Nov;3(6):684-7.
Previous work in our laboratory has demonstrated a simple, dynamic in vitro calcification method for studying bovine pericardial heart valves. The calcification produced closely resembled that found in clinical explant valves. The current study extends this technique to the porcine aortic bioprosthesis. Five Carpentier-Edwards porcine aortic bioprostheses were calcified in vitro in a modified wear tester. All valves calcified to a similar degree as bovine pericardial valves. Calcification predominated on the ribbed tissue structures near the commissures on the outflow surfaces. The same calcification pattern was seen in clinical explant valves. A number of anti-calcification modifications of porcine aortic valves were also investigated. These had all previously inhibited calcification of bovine pericardium in a rat subdermal implant model but had failed to reduce calcification in whole pericardial valves calcified in vitro under dynamic conditions. The modified porcine valves produced similar results: no modification achieved reduction of calcification on exposure to the functional valve calcification model. The dynamic in vitro calcification test has been shown to be useful for the study of both main types of bioprostheses, bovine pericardial and porcine aortic valves, and for the assessment of alterations to these biomaterials.
我们实验室之前的工作已经证明了一种用于研究牛心包心脏瓣膜的简单、动态的体外钙化方法。所产生的钙化与临床取出的瓣膜中发现的钙化非常相似。当前的研究将这项技术扩展到了猪主动脉生物假体。五个Carpentier-Edwards猪主动脉生物假体在改良的磨损试验机中进行体外钙化。所有瓣膜的钙化程度与牛心包瓣膜相似。钙化主要发生在流出表面连合处附近的带肋组织结构上。在临床取出的瓣膜中也观察到了相同的钙化模式。还研究了猪主动脉瓣膜的一些抗钙化修饰。这些修饰在大鼠皮下植入模型中均曾抑制牛心包的钙化，但在动态条件下体外钙化的完整心包瓣膜中未能减少钙化。改良后的猪瓣膜产生了类似的结果：在功能性瓣膜钙化模型中，没有一种修饰能实现钙化减少。体外动态钙化试验已被证明对研究两种主要类型的生物假体（牛心包和猪主动脉瓣膜）以及评估这些生物材料的改变很有用。
