Ferhat L, Ben-Ari Y, Khrestchatisky M
INSERM U. 29, 123, Paris, France.
C R Acad Sci III. 1994 Apr;317(4):304-9.
MAP2 proteins are among the major microtubule-associated proteins in the vertebrate nervous system. The high molecular weight proteins MAP2a, MAP2b and the low molecular weight protein MAP2c are encoded by distinct mRNAs generated by alternative splicing from the same primary transcript. MAP2c is primarily found in embryonic and neonatal brain. Using primers selected in the 5' and 3' non-coding regions of the rat MAP2c sequence and reverse transcription-coupled PCR from adult rat brain RNA, we have amplified and sequenced the entire coding region of a novel MAP2 isoform containing a 93 base pair insertion. Sequence analysis reveals that the additional exon encodes a domain that is highly homologous to repeats found in the 3' end of the coding region of MAP2, MAP4 and Tau mRNA sequences. In these microtubule-associated proteins, the domains encoded by the repeats are involved in microtubule binding and bundling.
微管相关蛋白2（MAP2）是脊椎动物神经系统中主要的微管相关蛋白之一。高分子量蛋白MAP2a、MAP2b和低分子量蛋白MAP2c由同一初级转录本通过可变剪接产生的不同mRNA编码。MAP2c主要存在于胚胎和新生大脑中。利用在大鼠MAP2c序列的5'和3'非编码区选择的引物以及从成年大鼠脑RNA进行的逆转录偶联PCR，我们扩增并测序了一种含有93个碱基对插入片段的新型MAP2亚型的整个编码区。序列分析表明，额外的外显子编码一个与MAP2、MAP4和Tau mRNA序列编码区3'端发现的重复序列高度同源的结构域。在这些微管相关蛋白中，由这些重复序列编码的结构域参与微管结合和成束。
