Bishop A, Paz M A, Gallop P M, Karnovsky M L
Department of Genetics, Harvard Medical School, Harvard School of Dental Medicine, Boston, MA.
Free Radic Biol Med. 1994 Oct;17(4):311-20. doi: 10.1016/0891-5849(94)90017-5.
PQQ, also called methoxatin, has been isolated from guinea-pig neutrophils. The organic cations diphenyleneiodonium (DPI) and diphenyliodonium (BPI) and the aromatic o-diamine 4,5-dimethylphenylenediamine (DIMPDA) sequester synthetic PQQ and inhibit its redox-cycling activity in a model system. Standards were made of adducts of tritiated PQQ with unlabeled DIMPDA and of unlabeled PQQ with tritiated DPI or DIMPDA. PQQ adducts were isolated from guinea-pig neutrophils with each of the tritiated inhibitors. They were separated and defined by high-performance liquid chromatography (HPLC). Tiron, a disodium benzene disulphonic acid, broke the DPI-PQQ adduct isolated from neutrophils and released free PQQ. Both DPI and DIMPDA, as well as BPI, blocked O2.- release by stimulated neutrophils. The blockade exerted by these inhibitors was released by the addition of PQQ to the cell suspensions. The data demonstrate the presence of PQQ in guinea-pig neutrophils and suggest that it has a possible role, direct or indirect, in the O2.(-)-producing respiratory burst.
吡咯喹啉醌（PQQ），也称为甲氧基噻唑啉酮，已从豚鼠中性粒细胞中分离出来。有机阳离子二亚苯基碘鎓（DPI）和二苯基碘鎓（BPI）以及芳香族邻二胺4,5-二甲基苯二胺（DIMPDA）能螯合合成的PQQ，并在模型系统中抑制其氧化还原循环活性。标准品由氚标记的PQQ与未标记的DIMPDA的加合物以及未标记的PQQ与氚标记的DPI或DIMPDA的加合物制成。用每种氚标记的抑制剂从豚鼠中性粒细胞中分离出PQQ加合物。通过高效液相色谱法（HPLC）对它们进行分离和鉴定。钛铁试剂，一种苯二磺酸二钠，能破坏从嗜中性粒细胞中分离出的DPI-PQQ加合物并释放出游离的PQQ。DPI和DIMPDA以及BPI均能阻断受刺激的中性粒细胞释放超氧阴离子（O2.-）。通过向细胞悬液中添加PQQ可解除这些抑制剂所产生的阻断作用。这些数据证明豚鼠中性粒细胞中存在PQQ，并表明它在产生超氧阴离子（O2.-）的呼吸爆发中可能直接或间接地发挥作用。
