尿胰蛋白酶抑制剂对羊膜有保护作用。

Urinary trypsin inhibitor has a protective effect on the amnion.

作者信息

el Maradny E, Kanayama N, Halim A, Maehara K, Terao T

机构信息

Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Japan.

出版信息

Gynecol Obstet Invest. 1994;38(3):169-72. doi: 10.1159/000292472.

DOI:10.1159/000292472

PMID:8001869

Abstract

Urinary trypsin inhibitor-related substance (UTI-R) was measured in the amniotic fluid (n = 30), first neonatal urine (n = 10), meconium (n = 10), and adult urine (n = 10). The concentration of UTI-R excreted in the neonatal urine was significantly higher than in adult urine and meconium. Positive immunostaining for UTI was observed in amnion, umbilical cord and placenta. The effect of UTI was studied on the amnion using fura-2-acetoxymethyl ester. Interleukin-1 beta (IL-1 beta) and tumor necrosis factor-beta (TNF-beta) led to a significant increase in relative fluorescence release (RFR) of fura-2 (p < 0.05 and p < 0.02, respectively) while endotoxin did not show significant changes of RFR of fura-2. The effect of IL-1 beta and TNF-beta was almost abolished after incubation of UTI with the amnion cells (p < 0.03). This suggests that UTI may have a protective effect on the amnion especially against IL-1 beta and TNF-beta.

摘要

在羊水（n = 30）、新生儿首次尿液（n = 10）、胎粪（n = 10）和成人尿液（n = 10）中检测尿胰蛋白酶抑制剂相关物质（UTI-R）。新生儿尿液中排泄的UTI-R浓度显著高于成人尿液和胎粪。在羊膜、脐带和胎盘中观察到UTI的阳性免疫染色。使用fura-2-乙酰氧基甲酯研究了UTI对羊膜的影响。白细胞介素-1β（IL-1β）和肿瘤坏死因子-β（TNF-β）导致fura-2的相对荧光释放（RFR）显著增加（分别为p < 0.05和p < 0.02），而内毒素未显示fura-2的RFR有显著变化。UTI与羊膜细胞孵育后，IL-1β和TNF-β的作用几乎被消除（p < 0.03）。这表明UTI可能对羊膜有保护作用，尤其是抵抗IL-1β和TNF-β。