人羊膜细胞中胎儿纤连蛋白产生的调节
Regulation of fetal fibronectin production in human amnion cells.
作者信息
Jackson G M, Edwin S S, Varner M W, Casal D, Mitchell M D
机构信息
Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City 84132, USA.
出版信息
J Soc Gynecol Investig. 1996 Mar-Apr;3(2):85-8. doi: 10.1016/1071-5576(95)00050-X.
OBJECTIVE
We evaluated the role of human amnion in the production of fetal fibronectin and assessed the regulation of fetal fibronectin production by inflammatory products and cytokines.
METHODS
Human amnion cells were grown in culture. At confluence, the cell were incubated with and without lipopolysaccharide, interleukin-1 beta, tumor necrosis factor-alpha, and interleukin-6. Fetal fibronectin production was measured in the supernatant fluid using an enzyme-linked immunosorbent assay technique.
RESULTS
Unstimulated amnion cells produced fetal fibronectin, and production was increased by lipopolysaccharide, interleukin-1 beta, tumor necrosis factor-alpha, and interleukin-6.
CONCLUSION
Human amnion cells in vitro produce fetal fibronectin in substantial quantities. This production is stimulated by inflammatory products and mediators that are considered to be important in the initiation of some cases of preterm labor.
目的
我们评估了人羊膜在胎儿纤连蛋白产生中的作用，并评估了炎症产物和细胞因子对胎儿纤连蛋白产生的调节作用。
方法
人羊膜细胞在培养中生长。汇合时，将细胞分别在有和无脂多糖、白细胞介素-1β、肿瘤坏死因子-α和白细胞介素-6的情况下进行孵育。使用酶联免疫吸附测定技术测量上清液中胎儿纤连蛋白的产生。
结果
未受刺激的羊膜细胞产生胎儿纤连蛋白，脂多糖、白细胞介素-1β、肿瘤坏死因子-α和白细胞介素-6可增加其产生。
结论
体外培养的人羊膜细胞大量产生胎儿纤连蛋白。这种产生受到炎症产物和介质的刺激，这些炎症产物和介质被认为在某些早产病例的启动中很重要。
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