Kumaravel S, Dharmalingam K
School of Biotechnology, Madurai Kamaraj University.
Indian J Biochem Biophys. 1994 Aug;31(4):280-7.
The chromosome of Streptomyces coelicolor A3(2) carries unstable DNA sequences hybridising with DNA sequences from an unstable chromosomal region of the related species S. lividans. These S. coelicolor sequences are nearly identical to those of S. lividans TK23 in organisation but differ from those of S. lividans 66 TK64 which harbours a tandem duplication of these sequences. Southern hybridisations using heterologous probes and S. coelicolor DNA cleaved with a variety of restriction enzymes permitted us to construct a partial restriction map of the unstable region of the chromosome of S. coelicolor. Genetic analysis shows that the unstable region yields distinguishable variants with several distinct DNA rearrangements.
天蓝色链霉菌A3(2)的染色体携带与相关物种变铅青链霉菌不稳定染色体区域的DNA序列杂交的不稳定DNA序列。这些天蓝色链霉菌序列在组织上与变铅青链霉菌TK23的序列几乎相同，但与含有这些序列串联重复的变铅青链霉菌66 TK64的序列不同。使用异源探针和用多种限制酶切割的天蓝色链霉菌DNA进行的Southern杂交使我们能够构建天蓝色链霉菌染色体不稳定区域的部分限制图谱。遗传分析表明，不稳定区域产生具有几种不同DNA重排的可区分变体。
