Tulman E R, Garmendia A E
Department of Pathobiology, University of Connecticut, Storrs.
Arch Virol. 1994;136(1-2):149-59. doi: 10.1007/BF01538824.
The infectivity of pseudorabies virus (PrV) was demonstrated in a cell substrate derived from swine skin explant cultures designated primary porcine skin cells (c/cSLA PPSC). c/cSLA PPSC infected with either wild type or TK- PrV strain Kaplan (Ka) developed typical cytopathologic changes (CPE) as early as 4 h post inoculation (p.i.). The CPE caused by PrV on c/cSLA PPSC was specifically neutralized by covalescent swine sera. Synthesis of late viral proteins was demonstrated in PrV-infected c/cSLA PPSC by indirect fluorescent antibody staining using monoclonal antibodies (mAbs) specific for PrV gIII. PrV induced protein synthesis was further confirmed by specific immunoprecipitation of 35S-methionine labeled viral polypeptides from PrV-infected c/cSLA PPSC with PrV convalescent swine serum, PrV immune mouse serum or mAb to PrV gIII. Moreover, the virus progeny derived from c/cSLA PPSC was shown to be infectious for MDBK cells and this infection was specifically neutralized by PrV convalescent swine serum. The capacity c/cSLA PPSC to support a complete growth cycle of PrV and the relative ease of deriving these cells from pigs can be applied in an autologous fashion in studies of cellular immunity where the MHC needs to be matched.
伪狂犬病病毒（PrV）的感染性在源自猪皮肤外植体培养物的细胞底物中得到证实，该底物被指定为原代猪皮肤细胞（c/cSLA PPSC）。感染野生型或TK- PrV株卡普兰（Ka）的c/cSLA PPSC早在接种后4小时（p.i.）就出现了典型的细胞病变效应（CPE）。PrV在c/cSLA PPSC上引起的CPE被康复猪血清特异性中和。通过使用针对PrV gIII的单克隆抗体（mAbs）进行间接荧光抗体染色，在PrV感染的c/cSLA PPSC中证实了晚期病毒蛋白的合成。通过用PrV康复猪血清、PrV免疫小鼠血清或针对PrV gIII的mAb从PrV感染的c/cSLA PPSC中特异性免疫沉淀35S-甲硫氨酸标记的病毒多肽，进一步证实了PrV诱导的蛋白质合成。此外，源自c/cSLA PPSC的病毒后代对MDBK细胞具有感染性，并且这种感染被PrV康复猪血清特异性中和。c/cSLA PPSC支持PrV完整生长周期的能力以及从猪中获取这些细胞的相对容易程度，可以以自体方式应用于需要匹配MHC的细胞免疫研究中。
